Journal: PLoS ONE

Article Title: Inflamed In Vitro Retina: Cytotoxic Neuroinflammation and Galectin-3 Expression

doi: 10.1371/journal.pone.0161723

Figure Lengend Snippet: Immunohistochemical staining of Gal-3 (red) expressing microglia. All microglia were detected using the microglia marker Iba1 (green). At 0 DIV no Gal-3-expressing cells were found (A, B). In controls, at 3, 4 and 7 DIV Iba1/Gal-3 co-expressing cells were found and only in the GCL (C, D, G, H, K, and L). LPS-treated retinas displayed larger numbers of Iba1/Gal-3 co-expressing cells that were located in the GCL, INL and OPL at 3, 4 and 7 DIV. Scale bar: 200 μm.

Article Snippet: Sections were then incubated with primary antibodies, rabbit anti-glial fibrillary acidic protein (GFAP, 1:2000, DAKO Cytomation, Glostrup, Denmark), rabbit anti-Iba1 (1:200, WAKO, Tokyo, Japan), rat anti-mouse ED1 (CD68, 1:1000, Nordic Biosite, Täby, Sweden), rat anti-mouse Gal-3 (1:500 WAKO, Tokyo, Japan), mouse anti-NeuN (1:100, Chemicon International, Temecula, CA, USA) or anti-goat Ki67 (1:100, Millipore, Temecula, CA, USA) at 4°C overnight, before subsequent rinsing and incubation in secondary antibodies for 2 h. Secondary antibodies used were Texas Red-conjugated donkey anti-rabbit antibody (1:200; Abcam, Cambridge, UK), Alexa Fluor 488 goat anti-rabbit IgG (Molecular Probes, Inc. Eugene, OR, USA) and Alexa Fluor 564 goat anti-rat (Molecular Probes, Inc. Eugene, OR, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Marker

Journal: PLoS ONE

Article Title: Inflamed In Vitro Retina: Cytotoxic Neuroinflammation and Galectin-3 Expression

doi: 10.1371/journal.pone.0161723

Figure Lengend Snippet: Quantification of fractions of cells expressing ED1 and Gal-3, respectively, of the total number of Iba1-stained cells. ANOVA analysis was performed for comparison between the LPS-treated group and the control groups. Data are expressed as mean±StDev (n = 3/group) *p<0.05, ***p<0.001.

Article Snippet: Sections were then incubated with primary antibodies, rabbit anti-glial fibrillary acidic protein (GFAP, 1:2000, DAKO Cytomation, Glostrup, Denmark), rabbit anti-Iba1 (1:200, WAKO, Tokyo, Japan), rat anti-mouse ED1 (CD68, 1:1000, Nordic Biosite, Täby, Sweden), rat anti-mouse Gal-3 (1:500 WAKO, Tokyo, Japan), mouse anti-NeuN (1:100, Chemicon International, Temecula, CA, USA) or anti-goat Ki67 (1:100, Millipore, Temecula, CA, USA) at 4°C overnight, before subsequent rinsing and incubation in secondary antibodies for 2 h. Secondary antibodies used were Texas Red-conjugated donkey anti-rabbit antibody (1:200; Abcam, Cambridge, UK), Alexa Fluor 488 goat anti-rabbit IgG (Molecular Probes, Inc. Eugene, OR, USA) and Alexa Fluor 564 goat anti-rat (Molecular Probes, Inc. Eugene, OR, USA).

Techniques: Expressing, Staining

Journal: Scientific Reports

Article Title: Berberine inhibits adipocyte differentiation, proliferation and adiposity through down-regulating galectin-3

doi: 10.1038/s41598-019-50103-5

Figure Lengend Snippet: Effects of BBR on Gal-3 promoter activity. A recombinant plasmid expressing luc2 reporter gene driven by the mouse Gal-3 promoter was co-tranfected with the pGL4.74[ hRluc /TK] Vector into mouse primary preadipocytes. After 24 h of transfection, cells were treated with DMSO or different concentrations of BBR for 24 h. ( A ) Alternatively, cells were induced for differentiation with MDI for 24 h after transfection, and meanwhile DMSO or BBR were added and incubated. ( B ) After treatment, the activities of firefly luciferase and Renilla luciferase were determined. After normalization to Renilla luciferase, Gal-3 promoter activities were presented as fold of control cells. Values are mean ± SD of 4 separate experiments. (A) * p < 0.05, ** p < 0.01 vs . that of DMSO. (B) ** p < 0.01 vs . that of preadipocytes; # p < 0.05, ## p < 0.01 vs . that of cells treated with MDI + DMSO.

Article Snippet: The recombinant Gal-3-pGL4.10[ luc2 ] plasmid was selected and amplified, and the correct insertion of Gal-3 gene promoter was verified by DNA sequencing (BGI Shenzhen, Shenzhen, China).

Techniques: Activity Assay, Recombinant, Plasmid Preparation, Expressing, Transfection, Incubation, Luciferase, Control